Four Tremadocianian sections of the Upper Yangtze Region of South China are intensively studied for biostratigraphical division and correlation. Based on the analysis of lithofacies, lithofacies associations, and facies successions, the detailed sea level curves of Tremadocian are revealed. Ten lithofacies which are grouped into three lithofacies associations deposited in the shallow subtidal, deep subtidal and shale basin zones. At least five major transgression events are recognized from each section in early TS.1a, late TS.1a, early TS.1c, late TS.1c and late TS.1d. The most major transgression event occurred in early TS.1c. Compared with other paleoplates, eustatic sea level changes might be the major controlling factor for the sea level fluctuations of the Upper Yangtze Region of South China.